Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA.

In this protocol, we introduce:

  1. the factors that affect the specificity of primers;
  2. the principle of MFEprimer and its settings;
  3. how to use the MFEprimer to examine the specificity of primers.

For details, please download the PDF version.