Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA.
In this protocol, we introduce:
- the factors that affect the specificity of primers;
- the principle of MFEprimer and its settings;
- how to use the MFEprimer to examine the specificity of primers.
For details, please download the PDF version.