MFEprimer-3.3#

Download from here: https://github.com/quwubin/MFEprimer-3.0/releases

New feature#

In case of multiplex PCR for next-generation sequencing (e.g., tNGS), add three flags for output amplicons in fasta format.

For input file, support two ways:#

1. a file with primers in fasta format: all primers in this file will be treated by mfeprimer as multiplex PCR reaction.
2. a file with primers in tsv format: each line has at least three items: name forward_seq reverse_seq. Items since 4th will be ignored and no space are allowed in name. Primer paires in each line will be treated by mfeprimer as a normal two primer pair PCR reaction.

For output file, support two ways:#

1. traditional text format.
2. output in a tsv format.

Improvement#

Compiled binaries for multiple platforms.