Download from here: https://github.com/quwubin/MFEprimer-3.0/releases
New feature #
In case of multiplex PCR for next-generation sequencing (e.g., tNGS), add three flags for output amplicons in fasta format.
For input file, support two ways: #
- a file with primers in fasta format: all primers in this file will be treated by mfeprimer as multiplex PCR reaction.
- a file with primers in tsv format: each line has at least three items: name forward_seq reverse_seq. Items since 4th will be ignored and no space are allowed in name. Primer paires in each line will be treated by mfeprimer as a normal two primer pair PCR reaction.
For output file, support two ways: #
- traditional text format.
- output in a tsv format.
Compiled binaries for multiple platforms.