Updates #

2025-08-22 18:04:43 #

• Add new app: Retrieve subsequence from FASTA. Sometimes, you may need to retrieve a subsequence from a FASTA file for further analysis (e.g., BLAST, GC analysis, etc). This app is for that purpose.

2025-07-25 13:40:35 #

• The network is up again.

2025-07-25 09:48:31 #

• Apologies, we have been experiencing network issues since last night and are currently investigating. Please wait patiently.

2025-07-25 00:33:11 #

• The network equipment in the park will be upgraded on ​Sunday, July 27th (Beijing Time)​, with internet services suspended from ​9:30 AM to 5:00 PM (Beijing Time)​.

2025-07-22 10:23:59 #

• MP-Ref fix: prevent bdb1/bdb2 being same as db.
• MP-Ref fix: correct primer coverage condition in sangerDesign.
• MP-Ref doc: add SMN1 repeat region guide and update documentation.

2025-07-21 21:54:57 #

• MP-Ref is online: Multiplex PCR Primer Deisgn for Reference Genomes. Our enhanced multiplex PCR primer design tool accommodates a wide range of applications, streamlining the design process for complex PCR setups. The old name is MP2.

2024-10-10 22:38:37 #

• Servers and domain name are back online.

2024-09-20 21:15:30 #

• Domain name still have work to do, please use IP address instead until it is ready.
  • MFEprimer-4.0: http://123.58.98.249:8088/
  • MFEprimer-3.1: http://123.58.98.249:8084/spec

2024-09-17 11:07:34 #

• Server maintenance: from 2024-09-18 8:00am to 2024-09-20 12:00pm. All servers will be down for maintenance. Our company is moving to a new office, we expect to be back online as soon as possible.

2024-07-18 16:27:51 #

• Improve candidate primer selection strategy for large amplicons., e.g., 5000 bp, to save memory and time.

2024-06-13 17:51:40 #

• Add two new databases for spec and mp2: Anser cygnoides domesticus (swan goose) and Tremarctos ornatus (spectacled bear).

2024-03-31 22:18:48 #

• Server is back online.

2024-03-30 22:36:21 #

• Server maintenance for 24 hours.

2024-03-03 11:30:51 #

• tips for whole region design;
• tips for long size product design.

2024-03-02 23:27:47 #

• Bug Fix: for very long regions, I had improperly set a parameter to determine the specificity of the amplicon.

2024-02-24 21:26:35 #

• Improvement: update MP2 example page.

2024-02-17 23:29:02 #

• Improvement help information for MP2.

2024-02-06 23:14:03 #

• Improvement: add 10 fish genomes for spec and mp2 upon request.

2024-01-19 09:38:13 #

• Improvement: add mouse genome (mm39) for spec and mp2 upon request.

2024-01-18 23:58:07 #

• Bug fixed: parse chromosome names.