Singleplex Primer Design with MP-Ref

Calendar July 21, 2025
Application, MP-Ref
Primer Design, Singleplex PCR, MP-Ref

This is an example of designing singleplex primers using MP-Ref (demo link).

  1. Select the target database;
  2. Enter the targets in BED format;
  3. Choose “Validation method” = “[Singleplex] Sanger sequencing”;. example 1 input
  4. Below are the results. example 1 result
Backward Simutaneously Amplify Two or More Genomes with MP-Ref
STR mode Forward