Singleplex PCR | MFEprimer

Singleplex Primer Design with MP-Ref

July 21, 2025

Application, MP-Ref

Primer Design, Singleplex PCR, MP-Ref

This is an example of designing singleplex primers using MP-Ref (demo link).

Select the target database;
Enter the targets in BED format;
Choose “Validation method” = “[Singleplex] Sanger sequencing”;.
Below are the results.