February 27, 2021
Introduction # We have a list of SNP rs numbers, like:
rs1002315756 rs1003815568 rs1004109382 rs1004635980 rs1008829651 And we want the genome positions for each SNPS, the results should like:
#chrom chromStart chromEnd name ref altCount alts shiftBases freqSourceCount minorAlleleFreq majorAllele minorAllele maxFuncImpact class ucscNotes _dataOffset _dataLen chr1 51453 51454 rs1004109382 C 1 T, 0 0 0 snv rareSome,rareAll, 409400514 36 chr1 51594 51599 rs1004635980 GGGGG 1 GGGG, 4 12 -inf,-inf,0.
February 16, 2021
Introduction # MPprimer: a program for reliable multiplex PCR primer design
BACKGROUND: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered.