About MPprimer

February 16, 2021
primer design, multiplex PCR, MFEprimer

Introduction # MPprimer: a program for reliable multiplex PCR primer design BACKGROUND: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered. ...